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Validation Data Gallery

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  • Western Blot (WB) analysis of rat retina tissue using RS1 Polyclonal antibody (24430-1-AP)

    WB analysis of rat retina using 24430-1-AP (same clone as 24430-1-PBS)

    rat retina tissue were subjected to SDS PAGE followed by western blot with 24430-1-AP (RS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24430-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    WB analysis of mouse eye using 24430-1-AP (same clone as 24430-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 24430-1-AP (RS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IHC staining of mouse eye using 24430-1-AP (same clone as 24430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IHC staining of mouse eye using 24430-1-AP (same clone as 24430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IHC staining of mouse eye using 24430-1-AP (same clone as 24430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24430-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IF Staining of mouse eye using 24430-1-AP (same clone as 24430-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RS1 antibody (24430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24430-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IF Staining of mouse eye using 24430-1-AP (same clone as 24430-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RS1 antibody (24430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24430-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IF Staining of mouse eye using 24430-1-AP (same clone as 24430-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse eye tissue using 24430-1-AP (RS1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24430-1-PBS in a different storage buffer formulation.

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RS1 Polyclonal antibody, PBS Only

RS1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA
Cat No. 24430-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Synonyms

XLRS1, Retinoschisin, retinoschisin 1, X linked juvenile retinoschisis protein, X-linked juvenile retinoschisis protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24430-1-PBS targets RS1 in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RS1 fusion protein Ag18885 相同性解析による交差性が予測される生物種
Full Name retinoschisin 1
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC141638
Gene Symbol RS1
Gene ID (NCBI) 6247
RRIDAB_2879545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15537
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RS1 is also named as XLRS1. RS1 can bind negatively charged membrane lipids, such as phosphatidylserine and phosphoinositides. RS1 is required for normal structure and function of the retina (PMID:19093009). It may play a role in cell-cell adhesion processes in the retina (PMID:27114531). RS1 is high expression in the retina (at protein level) (PMID:10915776, PMID:9326935). It is detected in the inner segment of the photoreceptors, the inner nuclear layer, the inner plexiform layer and the ganglion cell layer. At the macula, it is expressed in both the outer and inner nuclear layers and in the inner plexiform layer (PMID:10915776, PMID:10915776). And RS1 is undetectable in the inner plexiform layers and the inner nuclear layer (PMID:10915776)