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RSAD2 Polyclonal antibody

RSAD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 28089-1-AP

Synonyms

2510004L01Rik, cig33, cig5, RSAD2, vig1, Viperin


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Tested Applications

Positive WB detected infetal human brain tissue, A549 cells, mouse spinal cord tissue
Positive IHC detected inmouse stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28089-1-AP targets RSAD2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSAD2 fusion protein Ag27733 相同性解析による交差性が予測される生物種
Full Name radical S-adenosyl methionine domain containing 2
Calculated molecular weight 361 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC017969
Gene symbol RSAD2
Gene ID (NCBI) 91543
RRIDAB_2881057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon.

Protocols

Product Specific Protocols
WB protocol for RSAD2 antibody 28089-1-APDownload protocol
IHC protocol for RSAD2 antibody 28089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Rubella Virus Triggers Type I Interferon Antiviral Response in Cultured Human Neural Cells: Involvement in the Control of Viral Gene Expression and Infectious Progeny Production

Authors - Sayuri Sakuragi
humanIHC

Am J Cancer Res

Identification of potential immune-related circRNA-miRNA-mRNA regulatory network in cutaneous squamous cell carcinoma

Authors - Yuanfeng Wei
humanWB

Cell Mol Biol Lett

5-Methoxyflavone-induced AMPKα activation inhibits NF-κB and P38 MAPK signaling to attenuate influenza A virus-mediated inflammation and lung injury in vitro and in vivo

Authors - Sushan Yang
mouseWB

Front Nutr

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics.

Authors - Qiongxin Liang
mouseWB

J Appl Toxicol

Intratracheal instillation of graphene oxide decreases anti-virus responses and lipid contents via suppressing Toll-like receptor 3 in mouse livers.

Authors - Xiaomin Tang
humanWB,IF,IP

Biochemistry

Interactions between Viperin, Vesicle-Associated Membrane Protein A, and Hepatitis C Virus Protein NS5A Modulate Viperin Activity and NS5A Degradation.

Authors - Soumi Ghosh