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RSAD2 Polyclonal antibody
RSAD2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (2)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 28089-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue, A549 cells, mouse spinal cord tissue |
Positive IHC detected in | mouse stomach tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 17 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
28089-1-AP targets RSAD2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Cited Reactivity | human, mouse, canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RSAD2 fusion protein Ag27733 相同性解析による交差性が予測される生物種 |
Full Name | radical S-adenosyl methionine domain containing 2 |
Calculated molecular weight | 361 aa, 42 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC017969 |
Gene symbol | RSAD2 |
Gene ID (NCBI) | 91543 |
RRID | AB_2881057 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon.
Protocols
Product Specific Protocols | |
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WB protocol for RSAD2 antibody 28089-1-AP | Download protocol |
IHC protocol for RSAD2 antibody 28089-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Mol Sci Rubella Virus Triggers Type I Interferon Antiviral Response in Cultured Human Neural Cells: Involvement in the Control of Viral Gene Expression and Infectious Progeny Production | ||
Am J Cancer Res Identification of potential immune-related circRNA-miRNA-mRNA regulatory network in cutaneous squamous cell carcinoma | ||
Cell Mol Biol Lett 5-Methoxyflavone-induced AMPKα activation inhibits NF-κB and P38 MAPK signaling to attenuate influenza A virus-mediated inflammation and lung injury in vitro and in vivo | ||
Front Nutr The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics. | ||
J Appl Toxicol Intratracheal instillation of graphene oxide decreases anti-virus responses and lipid contents via suppressing Toll-like receptor 3 in mouse livers. | ||
Biochemistry Interactions between Viperin, Vesicle-Associated Membrane Protein A, and Hepatitis C Virus Protein NS5A Modulate Viperin Activity and NS5A Degradation. |