Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HL-60 cells, PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
25190-1-AP targets RSL24D1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RSL24D1 fusion protein Ag18195 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal L24 domain containing 1 |
| Calculated molecular weight | 163 aa, 20 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC008409 |
| Gene Symbol | RSL24D1 |
| Gene ID (NCBI) | 51187 |
| RRID | AB_2879950 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHA3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RSL24D1 antibody 25190-1-AP | Download protocol |
| IF protocol for RSL24D1 antibody 25190-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly. | ||
mBio Genome-Scale CRISPR Screening Reveals Host Factors Required for Ribosome Formation and Viral Replication | ||
Cell The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity |