RSPH9 Polyclonal antibody
RSPH9 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 23253-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, NIH/3T3 cells, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 5 publications below |
Product Information
The immunogen of 23253-1-AP is RSPH9 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RSPH9 fusion protein Ag19745 相同性解析による交差性が予測される生物種 |
| Full Name | radial spoke head 9 homolog (Chlamydomonas) |
| Calculated molecular weight | 276 aa, 31 kDa |
| Observed molecular weight | 31-35 kDa |
| GenBank accession number | BC029519 |
| Gene symbol | RSPH9 |
| Gene ID (NCBI) | 221421 |
| RRID | AB_2879240 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RSPH9 antibody 23253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility | ||
Hum Mol Genet Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse. | ||
Eur J Hum Genet Bi-allelic variants in human TCTE1/DRC5 cause asthenospermia and male infertility. | ||
Cell Rep Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella | ||
EBioMedicine CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men | ||
Cell Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Célia (Verified Customer) (10-28-2024) | I tested 1/100 to be sure that it works in IF but the concentration was a little high so 1/200 should still be fine.
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