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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using RSPO3 Monoclonal antibody (66314-1-Ig)

    WB analysis of human skeletal muscle using 66314-1-Ig (same clone as 66314-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66314-1-Ig (RSPO3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using RSPO3 Monoclonal antibody (66314-1-Ig)

    WB analysis of A549 using 66314-1-Ig (same clone as 66314-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66314-1-Ig (RSPO3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RSPO3 Monoclonal antibody (66314-1-Ig)

    IHC staining of human lung cancer using 66314-1-Ig (same clone as 66314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66314-1-Ig (RSPO3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RSPO3 Monoclonal antibody (66314-1-Ig)

    IHC staining of human lung cancer using 66314-1-Ig (same clone as 66314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66314-1-Ig (RSPO3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RSPO3 Monoclonal antibody (66314-1-Ig)

    IF Staining of A549 using 66314-1-Ig (same clone as 66314-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RSPO3 antibody (66314-1-Ig, Clone: 1E4F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

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RSPO3 Monoclonal antibody, PBS Only

RSPO3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66314-1-PBS
Clone No.1E4F1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein with TSP type-1 repeat, Protein with TSP type 1 repeat, hRspo3, hPWTSR, CRISTIN1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66314-1-PBS targets RSPO3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RSPO3 fusion protein Ag9899 相同性解析による交差性が予測される生物種
Full Name R-spondin 3 homolog (Xenopus laevis)
Calculated molecular weight 272 aa, 31 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC022367
Gene Symbol RSPO3
Gene ID (NCBI) 84870
RRIDAB_2881695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BXY4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.