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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RSPO3 Monoclonal antibody, PBS Only

RSPO3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E4F1

Cat no : 66314-1-PBS

Synonyms

CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, PWTSR, R spondin 3, Roof plate specific spondin 3, RSPO3, THSD2

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using RSPO3 Monoclonal antibody (66314-1-Ig)

    WB analysis of human skeletal muscle using 66314-1-Ig (same clone as 66314-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66314-1-Ig (RSPO3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using RSPO3 Monoclonal antibody (66314-1-Ig)

    WB analysis of A549 using 66314-1-Ig (same clone as 66314-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66314-1-Ig (RSPO3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RSPO3 Monoclonal antibody (66314-1-Ig)

    IHC staining of human lung cancer using 66314-1-Ig (same clone as 66314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66314-1-Ig (RSPO3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RSPO3 Monoclonal antibody (66314-1-Ig)

    IHC staining of human lung cancer using 66314-1-Ig (same clone as 66314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66314-1-Ig (RSPO3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RSPO3 Monoclonal antibody (66314-1-Ig)

    IF Staining of A549 using 66314-1-Ig (same clone as 66314-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 66314-1-Ig(RSPO3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66314-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66314-1-PBS targets RSPO3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RSPO3 fusion protein Ag9899 相同性解析による交差性が予測される生物種
Full Name R-spondin 3 homolog (Xenopus laevis)
Calculated molecular weight 272 aa, 31 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC022367
Gene symbol RSPO3
Gene ID (NCBI) 84870
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.