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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

RTCD1 Polyclonal antibody

RTCD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15996-1-AP

Synonyms

RNA 3 phosphate cyclase, RNA cyclase, RPC, RPC1, RTC1, RTCD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RTCD1 Polyclonal antibody (15996-1-AP)

    WB analysis using 15996-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15996-1-AP (RTCD1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RTCD1 Polyclonal antibody (15996-1-AP)

    WB analysis of K-562 using 15996-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15996-1-AP (RTCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RTCD1 Polyclonal antibody (15996-1-AP)

    WB analysis of Jurkat using 15996-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15996-1-AP (RTCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RTCD1 Polyclonal antibody (15996-1-AP)

    IHC staining of mouse testis using 15996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15996-1-AP (RTCD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RTCD1 Polyclonal antibody (15996-1-AP)

    IHC staining of mouse testis using 15996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15996-1-AP (RTCD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells, THP-1 cells, U-251 cells, U-87 MG cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15996-1-AP targets RTCD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RTCD1 fusion protein Ag8904 相同性解析による交差性が予測される生物種
Full Name RNA terminal phosphate cyclase domain 1
Calculated molecular weight 379 aa, 41 kDa
Observed molecular weight 41-43 kDa
GenBank accession numberBC012604
Gene symbol RTCD1
Gene ID (NCBI) 8634
RRIDAB_2180923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RTCD1 antibody 15996-1-APDownload protocol
IHC protocol for RTCD1 antibody 15996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.

Authors - Irem Unlu
  • KD Validated
View Article