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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RTKN Polyclonal antibody

RTKN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12762-1-AP

Synonyms

rhotekin, RTKN, RTKN1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using RTKN Polyclonal antibody (12762-1-AP)

    WB analysis of A431 using 12762-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RTKN Polyclonal antibody (12762-1-AP)

    WB analysis of mouse brain using 12762-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using RTKN Polyclonal antibody (12762-1-AP)

    WB analysis of A431 using 12762-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RTKN Polyclonal antibody (12762-1-AP)

    WB analysis of rat brain using 12762-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12762-1-AP (RTKN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RTKN Polyclonal antibody (12762-1-AP)

    IHC staining of human prostate cancer using 12762-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12762-1-AP (RTKN antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inA431 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12762-1-AP targets RTKN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RTKN fusion protein Ag3265 相同性解析による交差性が予測される生物種
Full Name rhotekin
Calculated molecular weight 550 aa, 61 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC017727
Gene symbol RTKN
Gene ID (NCBI) 6242
RRIDAB_10644159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RTKN antibody 12762-1-APDownload protocol
IHC protocol for RTKN antibody 12762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols