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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RUFY2 Polyclonal antibody

RUFY2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19180-1-AP

Synonyms

KIAA1537, RABIP4R, RUFY2, ZFYVE13

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of mouse lung using 19180-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of mouse ovary using 19180-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of PC-3 using 19180-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of PC-3 using 19180-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of SH-SY5Y using 19180-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    IHC staining of human testis using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    IHC staining of human testis using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    IHC staining of human brain using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    IHC staining of human brain using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inmouse lung tissue, mouse ovary tissue, PC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19180-1-AP targets RUFY2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUFY2 fusion protein Ag5938 相同性解析による交差性が予測される生物種
Full Name RUN and FYVE domain containing 2
Calculated molecular weight 403 aa, 46 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC041092
Gene symbol RUFY2
Gene ID (NCBI) 55680
RRIDAB_10643382
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RUFY2 antibody 19180-1-APDownload protocol
IHC protocol for RUFY2 antibody 19180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols