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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of mouse lung using 19180-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis using 19180-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of SH-SY5Y using 19180-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of mouse ovary using 19180-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of PC-3 using 19180-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RUFY2 Polyclonal antibody (19180-1-AP)

    WB analysis of PC-3 using 19180-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    IHC staining of human testis using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    IHC staining of human testis using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    IHC staining of human brain using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RUFY2 Polyclonal antibody (19180-1-AP)

    IHC staining of human brain using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RUFY2 Polyclonal antibody

RUFY2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19180-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ZFYVE13, RUN and FYVE domain-containing protein 2, RABIP4R, Rab4-interacting protein related, KIAA1537

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, mouse lung tissue, mouse ovary tissue, SH-SY5Y cells, U-251 cells, rat lung tissue
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19180-1-AP targets RUFY2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUFY2 fusion protein Ag5938 相同性解析による交差性が予測される生物種
Full Name RUN and FYVE domain containing 2
Calculated molecular weight 403 aa, 46 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC041092
Gene Symbol RUFY2
Gene ID (NCBI) 55680
RRIDAB_10643382
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXA3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RUFY2 antibody 19180-1-APDownload protocol
IHC protocol for RUFY2 antibody 19180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols