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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RUFY3 Recombinant antibody, PBS Only (Detector)

RUFY3 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240519E7

Cat no : 83601-2-PBS

Synonyms

RIPx, Rap2-interacting protein x, Rap2 interacting protein x, Protein RUFY3, KIAA0871

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RUFY3 Recombinant antibody (83601-2-RR)

    WB analysis using 83601-2-RR (same clone as 83601-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83601-2-RR (RUFY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83601-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using RUFY3 Recombinant antibody (83601-2-RR)

    FC experiment of A431 using 83601-2-RR (same clone as 83601-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RUFY3 Recombinant antibody (83601-2-RR, Clone:240519E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83601-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00602-2

    Cytometric bead array standard curve of MP00602-2

    Cytometric bead array standard curve of MP00602-2, RUFY3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83601-1-PBS. Detection antibody: 83601-2-PBS. Standard: Ag35558. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83601-2-RR

    Affinity and Kinetic Characterization of 83601-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83601-2-RR against Human RUFY3 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83601-2-PBS targets RUFY3 as part of a matched antibody pair:

MP00602-2: 83601-1-PBS capture and 83601-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RUFY3 fusion protein Ag35558 相同性解析による交差性が予測される生物種
Full Name RUN and FYVE domain containing 3
Calculated molecular weight 53 kDa
GenBank accession numberBC051716
Gene symbol RUFY3
Gene ID (NCBI) 22902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.