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  • Featured Product
  • KD/KO Validated

RUNDC3A Polyclonal antibody

RUNDC3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20531-1-AP

Synonyms

Rap2 interacting protein 8, RAP2IP, RPIP 8, RPIP8, RUN domain containing 3A, RUNDC3A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RUNDC3A Polyclonal antibody (20531-1-AP)

    WB analysis using 20531-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20531-1-AP (RUNDC3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RUNDC3A Polyclonal antibody (20531-1-AP)

    IHC staining of mouse brain using 20531-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20531-1-AP (RUNDC3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RUNDC3A Polyclonal antibody (20531-1-AP)

    IHC staining of mouse brain using 20531-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20531-1-AP (RUNDC3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPC-12 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20531-1-AP targets RUNDC3A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUNDC3A fusion protein Ag14393 相同性解析による交差性が予測される生物種
Full Name RUN domain containing 3A
Calculated molecular weight 446 aa, 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC006194
Gene symbol RUNDC3A
Gene ID (NCBI) 10900
RRIDAB_10695757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RUNDC3A antibody 20531-1-APDownload protocol
IHC protocol for RUNDC3A antibody 20531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Discov

RUNDC3A regulates SNAP25-mediated chemotherapy resistance by binding AKT in gastric neuroendocrine carcinoma (GNEC).

Authors - Pengchen Chen
  • KO Validated
View Article