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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RUNDC3B Polyclonal antibody

RUNDC3B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17113-1-AP

Synonyms

RPIP 9, RPIB9, Rap2-interacting protein 9, Rap2-binding protein 9, Rap2 interacting protein 9

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using RUNDC3B Polyclonal antibody (17113-1-AP)

    WB analysis of MCF-7 using 17113-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17113-1-AP (RUNDC3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RUNDC3B Polyclonal antibody (17113-1-AP)

    WB analysis of mouse brain using 17113-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17113-1-AP (RUNDC3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RUNDC3B Polyclonal antibody (17113-1-AP)

    IF Staining of MCF-7 using 17113-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17113-1-AP (RUNDC3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF7 cells, mouse brain tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17113-1-AP targets RUNDC3B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUNDC3B fusion protein Ag10797 相同性解析による交差性が予測される生物種
Full Name RUN domain containing 3B
Calculated molecular weight 387 aa, 43 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC022520
Gene symbol RUNDC3B
Gene ID (NCBI) 154661
RRIDAB_10699872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RUNDC3B antibody 17113-1-APDownload protocol
IF protocol for RUNDC3B antibody 17113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols