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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RUNX1 (N-terminal) Polyclonal antibody

RUNX1 (N-terminal) Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 19555-1-AP

Synonyms

AML1, AML1 EVI 1, AMLCR1, CBF alpha 2, CBFA2, EVI 1, Oncogene AML 1, PEA2 alpha B, PEBP2 alpha B, PEBP2aB, RUNX1, RUNX1 (N-terminal)

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of mouse liver using 19555-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Sp2/0 cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of Sp2/0 using 19555-1-AP

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of Jurkat using 19555-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of HL-60 using 19555-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of MCF-7 using 19555-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of mouse spleen using 19555-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of SH-SY5Y using 19555-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of Raji using 19555-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of mouse thymus using 19555-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of HEK-293 using 19555-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of Raji using 19555-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Sp2/0 cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of Sp2/0 using 19555-1-AP

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Sp2/0 cells using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of Sp2/0 using 19555-1-AP

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of mouse liver using 19555-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    WB analysis of mouse liver using 19555-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using RUNX1 (N-terminal) Polyclonal antibody (19555-1-AP)

    IP experiment of mouse liver using 19555-1-AP

    IP result of anti-RUNX1 (N-terminal) (IP:19555-1-AP, 4ug; Detection:19555-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, HL-60 cells, Jurkat cells, MCF-7 cells, mouse spleen tissue, mouse thymus tissue, Raji cells, SH-SY5Y cells, Sp2/0 cells
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19555-1-AP targets RUNX1 (N-terminal) in WB, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name runt-related transcription factor 1
Calculated molecular weight 51 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberNM_001754
Gene symbol RUNX1
Gene ID (NCBI) 861
RRIDAB_10949190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa. This antibody (19555-1-AP) can't recognize 37 kDa isoform 11.

Protocols

Product Specific Protocols
WB protocol for RUNX1 (N-terminal) antibody 19555-1-APDownload protocol
IP protocol for RUNX1 (N-terminal) antibody 19555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Protein Expr Purif

Msx1 cooperates with Runx1 for inhibiting myoblast differentiation.

Authors - Guoqiang Zhou
View Article
humanWB,CoIP

Mol Cells

RUNX1 Ameliorates Rheumatoid Arthritis Progression through Epigenetic Inhibition of LRRC15

Authors - Hao Ding
View Article
ratChIP,WB

Respir Res

RUNX1 targeting AKT3 promotes alveolar hypercoagulation and fibrinolytic inhibition in LPS induced ARDS

Authors - Chuan Xiao
View Article