Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of ROS1728 using 20700-1-AP
ROS1728 cells were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 20700-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of ROS1728 using 20700-1-AP
IP result of anti-RUNX2 (IP:20700-1-AP, 4ug; Detection:20700-1-AP 1:1000) with ROS1728 cells lysate 2000 ug.
IHC staining of rat kidney using 20700-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20700-1-AP (RUNX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat skin using 20700-1-AP
Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 20700-1-AP (RUNX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of ROS1728 using 20700-1-AP
Immunofluorescent analysis of (4% PFA) fixed ROS1728 cells using RUNX2 antibody (20700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 20700-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RUNX2 (20700-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

RUNX2 Polyclonal antibody

RUNX2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 20700-1-AP

Publications(187)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Synonyms

OSF2, OSF 2, Core-binding factor subunit alpha-1, CBF-alpha-1, AML-3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (187)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	ROS1728 cells, MCF-7 cells
Positive IP detected in	ROS1728 cells
Positive IHC detected in	rat skin tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	ROS1728 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 163 publications below
IHC	See 26 publications below
IF	See 36 publications below
IP	See 2 publications below
ChIP	See 3 publications below

Product Information

20700-1-AP targets RUNX2 in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse, rat, pig, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	runt-related transcription factor 2
Calculated molecular weight	57 kDa
Observed molecular weight	57-60 kDa
GenBank accession number	NM_004348
Gene Symbol	RUNX2
Gene ID (NCBI)	860
RRID	AB_2722783
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13950
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

Protocols

Product Specific Protocols
WB protocol for RUNX2 antibody 20700-1-AP	Download protocol
IHC protocol for RUNX2 antibody 20700-1-AP	Download protocol
IF protocol for RUNX2 antibody 20700-1-AP	Download protocol
IP protocol for RUNX2 antibody 20700-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Bioact Mater 3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration Authors - Lei Zhang View Article
mouse	WB	Small Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy Authors - Dan Li View Article
mouse	WB	Redox Biol Endogenous hydrogen sulfide accelerated trauma-induced heterotopic ossification through the Ca2+/ERK pathway-enhanced aberrant osteogenic activity Authors - Zhengqiang Yuan View Article
human	WB	Mater Today Bio Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration. Authors - Zhi-Hu Zhao View Article
mouse	WB,IF	J Nanobiotechnology Ultrasound-triggered piezoelectric polyetheretherketone with boosted osteogenesis via regulating Akt/GSK3β/β-catenin pathway Authors - Yue Li View Article
	WB	ACS Appl Mater Interfaces Synergistic Effect of Magneto-Mechanical Bioengineered Stem Cells and Magnetic Field to Alleviate Osteoporosis Authors - Congcong Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of ROS1728 using 20700-1-AP

WB analysis using 20700-1-AP

IP experiment of ROS1728 using 20700-1-AP

IHC staining of rat kidney using 20700-1-AP

IHC staining of rat skin using 20700-1-AP

IF Staining of ROS1728 using 20700-1-AP

FC experiment of HepG2 using 20700-1-AP

RUNX2 Polyclonal antibody

RUNX2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration

Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy

Endogenous hydrogen sulfide accelerated trauma-induced heterotopic ossification through the Ca2+/ERK pathway-enhanced aberrant osteogenic activity

Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration.

Ultrasound-triggered piezoelectric polyetheretherketone with boosted osteogenesis via regulating Akt/GSK3β/β-catenin pathway

Synergistic Effect of Magneto-Mechanical Bioengineered Stem Cells and Magnetic Field to Alleviate Osteoporosis