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  • KD/KO Validated

RUVBL1 Polyclonal antibody

RUVBL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, CoIP, CHIP, ELISA

Conjugate

Unconjugated

Cat no : 10210-2-AP

Synonyms

49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A



Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RUVBL1 Ready-To-Use IHC Kit. RUVBL1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10210-2-AP targets RUVBL1 in WB, IP, IHC, IF, CoIP, CHIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUVBL1 fusion protein Ag0281 相同性解析による交差性が予測される生物種
Full Name RuvB-like 1 (E. coli)
Calculated molecular weight 456 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002993
Gene symbol RUVBL1
Gene ID (NCBI) 8607
RRIDAB_2184405
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.

Protocols

Product Specific Protocols
WB protocol for RUVBL1 antibody 10210-2-APDownload protocol
IHC protocol for RUVBL1 antibody 10210-2-APDownload protocol
IF protocol for RUVBL1 antibody 10210-2-APDownload protocol
IP protocol for RUVBL1 antibody 10210-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Nat Immunol

Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families.

Authors - Yi Zhong
mouseWB

Mol Cell

Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.

Authors - Sang Gyun Kim
mouseCHIP

Nat Struct Mol Biol

R loops regulate promoter-proximal chromatin architecture and cellular differentiation.

Authors - Poshen B Chen
humanWB

Sci Adv

Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase.

Authors - Fabian Zimmermann
  • KD Validated
humanWB

Sci Adv

Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.

Authors - Seung Ho Shin
  • KD Validated
humanIP

Genes Dev

SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA.

Authors - Ailone Tichon

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Deyong (Verified Customer) (03-11-2022)

It worked well in H1 human embryonic stem cell.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human embryonic stem cell