RWDD1 Polyclonal antibody
RWDD1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17036-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse thymus tissue, HeLa cells, Jurkat cells, rat thymus tissue |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
17036-1-AP targets RWDD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RWDD1 fusion protein Ag10525 相同性解析による交差性が予測される生物種 |
| Full Name | RWD domain containing 1 |
| Calculated molecular weight | 243 aa, 28 kDa |
| Observed molecular weight | 28-36 kDa |
| GenBank accession number | BC015802 |
| Gene symbol | RWDD1 |
| Gene ID (NCBI) | 51389 |
| RRID | AB_2184704 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RWDD1(RWD domain-containing protein 1) is also named as DFRP2(DRG family-regulatory protein 2) and belongs to the RWDD1/GIR2 family. RWDD1 is a thymus aging-related protein which contains an RWD domain at its N-terminus and it is expressed in both thymocytes and thymic epithelial cells and located primarily in the cytoplasm. This protein expression in thymus is decreased in aged and oxidatively stressed mice (PMID:18954556). RWDD1 has a calculated molecular mass of 28 kDa, and apparent molecular mass of 32-36 kDa due to its high acidic amino acid composition that results in the slowed migration behavior in electrophoresis (PMID: 30278355, 18954556).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RWDD1 antibody 17036-1-AP | Download protocol |
| IHC protocol for RWDD1 antibody 17036-1-AP | Download protocol |
| IP protocol for RWDD1 antibody 17036-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Endocrinol RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation. |
