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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ORF-3 Polyclonal antibody (10631-1-AP)

    WB analysis of HepG2 using 10631-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10631-1-AP (ORF-3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ORF-3 Polyclonal antibody (10631-1-AP)

    WB analysis of HepG2 using 10631-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10631-1-AP (RXRA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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ORF-3 Polyclonal antibody

ORF-3 Polyclonal Antibody for WB, ELISA
Cat No. 10631-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ORF3, ORF-3, U66306

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10631-1-AP targets ORF-3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ORF-3 fusion protein Ag0987 相同性解析による交差性が予測される生物種
Full Name retinoid X receptor, alpha
Calculated molecular weight 462 aa, 51 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC007925
Gene Symbol RXRA
Gene ID (NCBI) 6256
RRIDAB_2877738
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19793
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This gene shares the same gene ID with the RXRA, but the genetic encoding protein is different and this gene encodes the ORF-3 protein instead of RXRA. The function of ORF-3 is to be confirmed in the future and is unknown now.

Protocols

Product Specific Protocols
WB protocol for ORF-3 antibody 10631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols