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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RXRA Monoclonal antibody, PBS Only

RXRA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H6C4

Cat no : 60198-1-PBS

Synonyms

NR2B1, Retinoid X receptor alpha, retinoid X receptor, alpha, RXRA, RXRα

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RXRA Monoclonal antibody (60198-1-Ig)

    WB analysis of HeLa using 60198-1-Ig (same clone as 60198-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60198-1-Ig (RXRA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60198-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RXRA Monoclonal antibody (60198-1-Ig)

    IHC staining of human stomach cancer using 60198-1-Ig (same clone as 60198-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60198-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RXRA Monoclonal antibody (60198-1-Ig)

    IHC staining of mouse cerebellum using 60198-1-Ig (same clone as 60198-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60198-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RXRA Monoclonal antibody (60198-1-Ig)

    IHC staining of human stomach cancer using 60198-1-Ig (same clone as 60198-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60198-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using RXRA Monoclonal antibody (60198-1-Ig)

    IHC staining of rat liver using 60198-1-Ig (same clone as 60198-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60198-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RXRA Monoclonal antibody (60198-1-Ig)

    IHC staining of mouse cerebellum using 60198-1-Ig (same clone as 60198-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60198-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using RXRA Monoclonal antibody (60198-1-Ig)

    IHC staining of rat liver using 60198-1-Ig (same clone as 60198-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60198-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60198-1-PBS targets RXRA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RXRA fusion protein Ag0987 相同性解析による交差性が予測される生物種
Full Name retinoid X receptor, alpha
Calculated molecular weight 462 aa, 51 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC007925
Gene symbol RXRA
Gene ID (NCBI) 6256
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver, and also expressed in the lungs, kidneys, and heart. It can recognize the the mature 54 kDa RXRA and the truncated 44 kD RXRA (PMID: 20541701).