Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, mouse ovary tissue |
| Positive IHC detected in | rat eye tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
11129-1-AP targets RXRG in WB, IHC, IF, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RXRG fusion protein Ag1611 相同性解析による交差性が予測される生物種 |
| Full Name | retinoid X receptor, gamma |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 51-55 kDa |
| GenBank accession number | BC012063 |
| Gene Symbol | RXRG |
| Gene ID (NCBI) | 6258 |
| RRID | AB_2182635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48443 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RXRG, also named as, Retinoic acid receptor RXR-gamma, is a 463 amino acid protein, which localize in the nucleus and may form homodimer. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RXRG antibody 11129-1-AP | Download protocol |
| IHC protocol for RXRG antibody 11129-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Cholecalciferol (vitamin D3) has a direct protective activity against interleukin 6-induced atrophy in C2C12 myotubes. | ||
Am J Physiol Lung Cell Mol Physiol Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia. | ||
Cells EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP. |