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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using RYBP Polyclonal antibody (11365-1-AP)

    WB analysis of K-562 using 11365-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11365-1-AP (RYBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using RYBP Polyclonal antibody (11365-1-AP)

    IP experiment of mouse spleen using 11365-1-AP

    IP result of anti-RYBP (IP:11365-1-AP, 4ug; Detection:11365-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

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RYBP Polyclonal antibody

RYBP Polyclonal Antibody for WB, IP, ELISA
Cat No. 11365-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11365-1-AP targets RYBP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RYBP fusion protein Ag1908 相同性解析による交差性が予測される生物種
Full Name RING1 and YY1 binding protein
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC014959
Gene Symbol RYBP
Gene ID (NCBI) 23429
RRIDAB_2285461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N488
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]

Protocols

Product Specific Protocols
WB protocol for RYBP antibody 11365-1-APDownload protocol
IP protocol for RYBP antibody 11365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.

Authors - Xianchun Lan
View Article