- Featured Product
- KD/KO Validated
RYK Polyclonal antibody
RYK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 22138-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, A549 cells, mouse brain tissue, SH-SY5Y cells |
| Positive IHC detected in | human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
22138-1-AP targets RYK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RYK fusion protein Ag17540 相同性解析による交差性が予測される生物種 |
| Full Name | RYK receptor-like tyrosine kinase |
| Calculated molecular weight | 610 aa, 68 kDa |
| Observed molecular weight | 67-75 kDa |
| GenBank accession number | BC153090 |
| Gene symbol | RYK |
| Gene ID (NCBI) | 6259 |
| RRID | AB_11232602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tyrosine-protein kinase RYK may be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A, and WNT5A. It is involved in neuron differentiation, axon guidance, corpus callosum establishment, and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region, allowing the C-terminal intracellular product to translocate from the cytoplasm to the nucleus, which plays a crucial role in neuronal development.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RYK antibody 22138-1-AP | Download protocol |
| IHC protocol for RYK antibody 22138-1-AP | Download protocol |
| IF protocol for RYK antibody 22138-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neuroinflammation Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model | ||
Cell Mol Biol Lett Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway | ||
Pharmaceuticals (Basel) RYK Gene Expression Associated with Drug Response Variation of Temozolomide and Clinical Outcomes in Glioma Patients |
