RYR1 Polyclonal antibody
RYR1 Polyclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 26968-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse brain tissue, human cerebellum tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse cerebellum tissue, mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
26968-1-AP targets RYR1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RYR1 fusion protein Ag25120 相同性解析による交差性が予測される生物種 |
Full Name | ryanodine receptor 1 (skeletal) |
Calculated molecular weight | 565 kDa |
GenBank accession number | NM_000540 |
Gene symbol | RYR1 |
Gene ID (NCBI) | 6261 |
RRID | AB_2880703 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.
Protocols
Product Specific Protocols | |
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IHC protocol for RYR1 antibody 26968-1-AP | Download protocol |
IF protocol for RYR1 antibody 26968-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
JCI Insight Muscle specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism |