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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using RYR1 Monoclonal antibody (66539-1-Ig)

    IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using RYR1 Monoclonal antibody (66539-1-Ig)

    IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RYR1 Monoclonal antibody (66539-1-Ig)

    IHC staining of mouse cerebellum using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A-673 cells using RYR1 Monoclonal antibody (66539-1-Ig)

    IF Staining of A-673 using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A-673 cells using RYR1 antibody (66539-1-Ig, Clone: 1B4E12 ) at dilution of 1:300 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

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RYR1 Monoclonal antibody, PBS Only

RYR1 Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 66539-1-PBS
Clone No.1B4E12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

SKRR, RYR1/2/3, RYDR, Ryanodine receptor 1, CCO

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66539-1-PBS targets RYR1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RYR1 fusion protein Ag25120 相同性解析による交差性が予測される生物種
Full Name ryanodine receptor 1 (skeletal)
Calculated molecular weight 565 kDa
GenBank accession numberNM_000540
Gene Symbol RYR1
Gene ID (NCBI) 6261
RRIDAB_2881901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21817
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.