RYR2 Polyclonal antibody

RYR2 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 19765-1-AP

Synonyms

ryanodine receptor 2, cardiac, ryanodine receptor 2 (cardiac), hRYR-2, Cardiac muscle ryanodine receptor-calcium release channel, ARVD2


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Tested Applications

Positive IHC detected inhuman heart tissue, human brain tissue, human placenta tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19765-1-AP targets RYR2 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ryanodine receptor 2 (cardiac)
Calculated molecular weight 565 kDa
GenBank accession numberNM_001035
Gene symbol RYR2
Gene ID (NCBI) 6262
RRIDAB_10637859
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP). This antibody detects a band at ~250 kDa which has been reported (PMID: 22962011).

Protocols

Product Specific Protocols
IHC protocol for RYR2 antibody 19765-1-APDownload protocol
IF protocol for RYR2 antibody 19765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Cell Mol Life Sci

Zbtb16 increases susceptibility of atrial fibrillation in type 2 diabetic mice via Txnip-Trx2 signaling

Authors - Zhi-Xing Wei
rat

Part Fibre Toxicol

Oxygenated polycyclic aromatic hydrocarbons from ambient particulate matter induce electrophysiological instability in cardiomyocytes.

Authors - Sujin Ju

Philos Trans R Soc Lond B Biol Sci

Patient-specific induced pluripotent stem cell properties implicate Ca2+-homeostasis in clinical arrhythmia associated with combined heterozygous RYR2 and SCN10A variants

Authors - Yafei Zhou
mouse

Free Radic Biol Med

Loss of Camk2n1 aggravates cardiac remodeling and malignant ventricular arrhythmia after myocardial infarction in mice via NLRP3 inflammasome activation.

Authors - Zhixing Wei
rat

Basic Res Cardiol

Protective role of heme oxygenase-1 in atrial remodeling.

Authors - Yung-Hsin Yeh
rat

Cardiovasc Diabetol

PMID: 31558158

Authors - Yi-Hsin Chan