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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-30000

Synonyms

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

Validation Data Gallery

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  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite®555-conjugated Rabbit IgG control Polyclo (CL555-30000)

    FC experiment of NIH/3T3 using CL555-30000

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug CoraLite®555 Anti-Rabbit IgG control (CL555-30000) (red), control used were unstained NIH/3T3 cells (Blue). Positive control is CoraLite®555 Calreticulin (CL555-27298) (Green). Cells were fixed with permeabilized with Transcription Factor Staining Buffer Kit (PF00011). and permeabilized Flow Perm Buffer (PF00011).

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®555-conjugated Rabbit IgG control Polyclo (CL555-30000)

    FC experiment of MCF-7 using CL555-30000

    1X10^6 MCF-7 cells were surface stained with 0.4 ug CoraLite®555 Anti-Rabbit IgG control (CL555-30000), or 0.4 ug CoraLite®555-conjugated EPCAM Rabbit PolyAb (CL555-21050), or unstained (blue). Cells were not fixed.

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Tested Applications

Positive FC (Intra) detected inNIH/3T3 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-30000 targets Rabbit IgG control in FC, FC (Intra) applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name Rabbit IgG control
Gene symbol
Gene ID (NCBI)
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Protocols

Product Specific Protocols
FC protocol for CL555 Rabbit IgG control antibody CL555-30000Download protocol
Standard Protocols
Click here to view our Standard Protocols