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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC-conjugated Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Applications

FC (Intra)

Conjugate

FITC Fluorescent Dye

Cat no : FITC-30000

Synonyms

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG control, Rabbit IgG isotype, Rabbit Immunoglobulin, Rabbit Immunoglobulin, RIGGCON

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using FITC-conjugated Rabbit IgG control Polyclonal anti (FITC-30000)

    FC experiment of HeLa using FITC-30000

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug FITC Anti-Rabbit IgG control (FITC-30000) (red), or 0.4 ug FITC-conjugated ATF4 Rabbit PolyAb (FITC-10835) (green). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-30000 targets Rabbit IgG control in FC (Intra) applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name Rabbit IgG control
Gene symbol
Gene ID (NCBI)
RRIDAB_2920492
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths498 nm / 526 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Protocols

Product Specific Protocols
FC protocol for FITC Rabbit IgG control antibody FITC-30000Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Virulence

Exploring the modulatory impact of isosakuranetin on Staphylococcus aureus: Inhibition of sortase A activity and α-haemolysin expression

Authors - Lili Tian
View Article