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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Rad51D Polyclonal antibody

Rad51D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11126-1-AP

Synonyms

HsTRAD, R51H3, RAD51 homolog D, RAD51 like 3 (S. cerevisiae), RAD51 like protein 3, Rad51D, RAD51L3, Trad

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Rad51D Polyclonal antibody (11126-1-AP)

    WB analysis using 11126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11126-1-AP (Rad51D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Rad51D Polyclonal antibody (11126-1-AP)

    IHC staining of human lymphoma using 11126-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11126-1-AP (Rad51D antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, A2780 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11126-1-AP targets Rad51D in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rad51D fusion protein Ag1595 相同性解析による交差性が予測される生物種
Full Name RAD51-like 3 (S. cerevisiae)
Calculated molecular weight 35 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC014422
Gene symbol Rad51D
Gene ID (NCBI) 5892
RRIDAB_10644480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAD51L3, also named RAD51D, R51H3 and TRAD, belongs to the recA family and RAD51 subfamily. It is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA, and specifically to nicks in duplex DNA. The Heterodimer of RAD51L3 is about 70 kDa.

Protocols

Product Specific Protocols
WB protocol for Rad51D antibody 11126-1-APDownload protocol
IHC protocol for Rad51D antibody 11126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols