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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Rad51D Polyclonal antibody (30068-1-AP)

    WB analysis using 30068-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30068-1-AP (Rad51D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Rad51D Polyclonal antibody (30068-1-AP)

    WB analysis of mouse brain using 30068-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 30068-1-AP (Rad51D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Rad51D Polyclonal antibody

Rad51D Polyclonal Antibody for WB, ELISA
Cat No. 30068-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Synonyms

HsTRAD, R51H3, RAD51 homolog D, RAD51 like 3 (S. cerevisiae), RAD51 like protein 3, Rad51D, RAD51L3, Trad

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells, A2780 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30068-1-AP targets Rad51D in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rad51D fusion protein Ag32303 相同性解析による交差性が予測される生物種
Full Name RAD51-like 3 (S. cerevisiae)
Calculated molecular weight 35 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC014422
Gene Symbol Rad51D
Gene ID (NCBI) 5892
RRIDAB_2935508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAD51L3, also named RAD51D, R51H3 and TRAD, belongs to the recA family and RAD51 subfamily. It is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA, and specifically to nicks in duplex DNA. The Heterodimer of RAD51L3 is about 70 kDa.

Protocols

Product Specific Protocols
WB protocol for Rad51D antibody 30068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols