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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RanGAP1 Polyclonal antibody

RanGAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27405-1-AP

Synonyms

SD, Ran GTPase-activating protein 1, KIAA1835

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RanGAP1 Polyclonal antibody (27405-1-AP)

    WB analysis using 27405-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27405-1-AP (RanGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using RanGAP1 Polyclonal antibody (27405-1-AP)

    WB analysis of SKOV-3 using 27405-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RanGAP1 Polyclonal antibody (27405-1-AP)

    WB analysis of MCF-7 using 27405-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RanGAP1 Polyclonal antibody (27405-1-AP)

    WB analysis of HEK-293 using 27405-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using RanGAP1 Polyclonal antibody (27405-1-AP)

    WB analysis of Neuro-2a using 27405-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RanGAP1 Polyclonal antibody (27405-1-AP)

    IHC staining of human stomach using 27405-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27405-1-AP (RanGAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, Neuro-2a cells, SKOV-3 cells, NIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27405-1-AP targets RanGAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RanGAP1 fusion protein Ag26128 相同性解析による交差性が予測される生物種
Full Name Ran GTPase activating protein 1
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa, 82 kDa
GenBank accession numberBC014044
Gene symbol RANGAP1
Gene ID (NCBI) 5905
RRIDAB_2880863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.

Protocols

Product Specific Protocols
WB protocol for RanGAP1 antibody 27405-1-APDownload protocol
IHC protocol for RanGAP1 antibody 27405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Authors - James W Clancy
View Article
mouseWB,IF

iScience

Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain

Authors - Terry R Suk
View Article