Rat IL-10 ELISA Kit

Sensitivity

2.1 pg/mL

Range

62.5-4000 pg/ml

Reactivity

Rat

Cat no : KE20003

Synonyms

Il10, IL-10, IL10X, interleukin 10



Product Information

KE20003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous rat IL10. The assay recognizes rat IL10. An antibody specific for rat IL10 has been pre-coated onto the microwells. The rat IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat IL10 is added to detect the captured rat IL10 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Rat IL-10 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 2.1 pg/mL
Range 62.5-4000 pg/ml
Reactivity Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 25325

Recovery

Sample TypeAverageRange
Serum107%96%-118%
Cell culture supernatants98%82%-115%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120110.89.38.4
220423.629.36.9
3201736.286.65.0

InterAssay

Samplenmean ( pg/mL)SDCV%
124463.427.55.9
224877.658.16.6
3241726.786.75.0

Background Information

Interleukin 10 is an essential anti-inflammatory cytokine that plays important roles as a negative regulator of immune responses to microbial antigens. IL10 is mainly secreted by macrophages, but also by T helper 1 (Th1) and Th2 lymphocytes, dendritic cells, cytotoxic T cells, B lymphocytes, monocytes and mast cells. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10 activity is mediated by the IL10 receptor (IL10R) which is a member of the class II cytokine receptor family.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il10, IL-10, IL10X, interleukin 10

Publications

SpeciesSample TypeTitle
ratcell culture supernatants

Biomaterials

Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.

Authors - Xianzhen Dong
rattissue homogenates

Phytother Res

Epigallocatechin-3-gallate in combination with corticosteroids mitigates heat stress-induced acute kidney injury through modulating heat shock protein 70 and toll-like receptor 4-dependent pathways

Authors - Faten Shafeek
ratserum

J Inflamm Res

Atractylone Alleviates Ethanol-Induced Gastric Ulcer in Rat with Altered Gut Microbiota and Metabolites

Authors - Ling Li
ratcell culture supernatants

J Cell Mol Med

NEDD4 attenuates phosgene-induced acute lung injury through the inhibition of Notch1 activation.

Authors - Yiru Shao
rattissue homogenates

World J Stem Cells

Intratracheal administration of umbilical cord-derived mesenchymal stem cells attenuates hyperoxia-induced multi-organ injury via heme oxygenase-1 and JAK/STAT pathways

Authors - Na Dong
ratserum,cell culture supernatants

Mol Immunol

Rapamycin treated tol-dendritic cells derived from BM-MSCs reversed graft rejection in a rat liver transplantation model by inducing CD8+CD45RC-Treg.

Authors - Lin Zhou