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Validation Data Gallery

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  • Western Blot (WB) analysis of pig retina tissue using Recoverin Monoclonal antibody (66521-1-Ig)

    WB analysis of pig retina using 66521-1-Ig (same clone as 66521-1-PBS)

    pig retina tissue were subjected to SDS PAGE followed by western blot with 66521-1-Ig (Recoverin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66521-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat retina tissue using Recoverin Monoclonal antibody (66521-1-Ig)

    WB analysis of rat retina using 66521-1-Ig (same clone as 66521-1-PBS)

    rat retina tissue were subjected to SDS PAGE followed by western blot with 66521-1-Ig (Recoverin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66521-1-PBS in a different storage buffer formulation.

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Recoverin Monoclonal antibody, PBS Only

Recoverin Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66521-1-PBS
Clone No.3B8E8

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig, mouse

Applications

WB, Indirect ELISA

Synonyms

Protein CAR, RCV1, RCVRN, recoverin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66521-1-PBS targets Recoverin in WB, Indirect ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Reactivity Human, rat, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recoverin fusion protein Ag0119 相同性解析による交差性が予測される生物種
Full Name recoverin
Calculated molecular weight 24 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC001720
Gene Symbol Recoverin
Gene ID (NCBI) 5957
RRIDAB_2881884
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35243
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Recoverin, belonging to a family of the neuronal calcium sensor (NCS) proteins, has a restricted expression in retinal photoreceptors or neurons or neuroendocrine cells. It has been suggested to play a role in light and dark adaptation by regulating rhodopsin phosphorylation. Recently, it has been found that autoantibodies against recoverin (24 kDa) have been strongly associated with cancer -associated retinopathy (CAR) syndrome, a paraneoplastic disease of the retina. But functions of recoverin in cancer cells remain unknown.