Red fluorescent protein Monoclonal antibody

Red fluorescent protein Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Recombinant Protein and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E3C5

Cat no : 67378-1-Ig

Synonyms

eqFP611, GFP-like chromoprotein, Red fluorescent protein eqFP611, RFP, RFP tag



Tested Applications

Positive WB detected inag27669

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67378-1-Ig targets Red fluorescent protein in WB, IF, ELISA applications and shows reactivity with Recombinant Protein samples.

Tested Reactivity Recombinant Protein
Cited Reactivity yeast, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Red fluorescent protein fusion protein Ag27669 相同性解析による交差性が予測される生物種
Full Name Red fluorescent protein
Calculated molecular weight 26 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_2882625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is a mouse (IgG2b) monoclonal antibody raised against RFP (eqFP611). It can be used to detect RFPs from eqFP611 and eqFP578 lineages.

Protocols

Product Specific Protocols
WB protocol for Red fluorescent protein antibody 67378-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
yeastWB

bioRxiv

Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins

Authors - Alaina H Willet
mouseIF

EMBO Rep

Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome

Authors - Fakun Cao
yeastWB

Mol Biol Cell

Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins

Authors - Alaina H Willet
mouseWB

Int J Mol Sci

Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1

Authors - Kazuhiro Hasegawa
WB

Mol Plant Pathol

Reticulons 3 and 6 interact with viral movement proteins

Authors - Jens Tilsner
WB

Autophagy

The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple

Authors - Runmin Che