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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RhoGDI Monoclonal antibody (66480-1-Ig)

    WB analysis using 66480-1-Ig (same clone as 66480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66480-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RhoGDI Monoclonal antibody (66480-1-Ig)

    WB analysis of HEK-293 using 66480-1-Ig (same clone as 66480-1-PBS)

    WB result of RhoGDI antibody (66480-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RhoGDI transfected HEK-293 cells. This data was developed using the same antibody clone with 66480-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RhoGDI Monoclonal antibody (66480-1-Ig)

    WB analysis using 66480-1-Ig (same clone as 66480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66480-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

RhoGDI Monoclonal antibody, PBS Only

RhoGDI Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66480-1-PBS
Clone No.1B1G9

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Synonyms

aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66480-1-PBS targets RhoGDI in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RhoGDI fusion protein Ag0791 相同性解析による交差性が予測される生物種
Full Name Rho GDP dissociation inhibitor (GDI) alpha
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC005851
Gene Symbol RhoGDI
Gene ID (NCBI) 396
RRIDAB_2881846
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52565
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.