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  • Featured Product
  • KD/KO Validated

RhoGDI Monoclonal antibody, PBS Only

RhoGDI Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1G9

Cat no : 66480-1-PBS

Synonyms

aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RhoGDI Monoclonal antibody (66480-1-Ig)

    WB analysis using 66480-1-Ig (same clone as 66480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66480-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RhoGDI Monoclonal antibody (66480-1-Ig)

    WB analysis of HEK-293 using 66480-1-Ig (same clone as 66480-1-PBS)

    WB result of RhoGDI antibody (66480-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RhoGDI transfected HEK-293 cells. This data was developed using the same antibody clone with 66480-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RhoGDI Monoclonal antibody (66480-1-Ig)

    WB analysis using 66480-1-Ig (same clone as 66480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66480-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66480-1-PBS targets RhoGDI in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RhoGDI fusion protein Ag0791 相同性解析による交差性が予測される生物種
Full Name Rho GDP dissociation inhibitor (GDI) alpha
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC005851
Gene symbol ARHGDIA
Gene ID (NCBI) 396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.