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  • Featured Product
  • KD/KO Validated

Ribosomal protein L4 Polyclonal antibody

Ribosomal protein L4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30182-1-AP

Synonyms

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ribosomal protein L4 Polyclonal antibody (30182-1-AP)

    WB analysis using 30182-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30182-1-AP (Ribosomal protein L4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Ribosomal protein L4 Polyclonal antibody (30182-1-AP)

    WB analysis of HeLa using 30182-1-AP

    WB result of Ribosomal protein L4 antibody (30182-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Ribosomal protein L4 Polyclonal antibody (30182-1-AP)

    IHC staining of human stomach cancer using 30182-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30182-1-AP (Ribosomal protein L4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ribosomal protein L4 Polyclonal antibody (30182-1-AP)

    IF Staining of HepG2 using 30182-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (30182-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30182-1-AP targets Ribosomal protein L4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ribosomal protein L4 fusion protein Ag32272 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L4
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC009888
Gene symbol Ribosomal protein L4
Gene ID (NCBI) 6124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

Protocols

Product Specific Protocols
WB protocol for Ribosomal protein L4 antibody 30182-1-APDownload protocol
IHC protocol for Ribosomal protein L4 antibody 30182-1-APDownload protocol
IF protocol for Ribosomal protein L4 antibody 30182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols