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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ribosomal protein L4 Monoclonal antibody, PBS Only

Ribosomal protein L4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C7A8

Cat no : 67028-1-PBS

Synonyms

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ribosomal protein L4 Monoclonal antibody (67028-1-Ig)

    WB analysis using 67028-1-Ig (same clone as 67028-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Ribosomal protein L4 Monoclonal antibody (67028-1-Ig)

    WB analysis of HeLa using 67028-1-Ig (same clone as 67028-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Ribosomal protein L4 Monoclonal antibody (67028-1-Ig)

    IHC staining of human lymphoma using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Ribosomal protein L4 Monoclonal antibody (67028-1-Ig)

    IHC staining of human lymphoma using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ribosomal protein L4 Monoclonal antibody (67028-1-Ig)

    IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ribosomal protein L4 Monoclonal antibody (67028-1-Ig)

    IF Staining of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Ribosomal protein L4 Monoclonal antibody (67028-1-Ig)

    FC experiment of HepG2 using 67028-1-Ig (same clone as 67028-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (67028-1-Ig, Clone:3C7A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67028-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67028-1-PBS targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ribosomal protein L4 fusion protein Ag28728 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L4
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009888
Gene symbol Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_2882343
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.