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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Rootletin Polyclonal antibody (55485-1-AP)

    WB analysis of mouse brain using 55485-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55485-1-AP (Rootletin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Rootletin Polyclonal antibody (55485-1-AP)

    IF Staining of hTERT-RPE1 using 55485-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Rootletin antibody (55485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Rootletin Polyclonal antibody

Rootletin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 55485-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CROCC, KIAA0445, ROLT, Rootletin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55485-1-AP targets Rootletin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ciliary rootlet coiled-coil, rootletin
Calculated molecular weight 229 kDa
Observed molecular weight228 kDa
GenBank accession numberNM_014675
Gene Symbol Rootletin
Gene ID (NCBI) 9696
RRIDAB_2935543
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5TZA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rootletin is predicted to enable kinesin binding activity and structural molecule activity. Involved in several processes, including centriole-centriole cohesion; positive regulation of cilium assembly; and positive regulation of protein localization to cilium.

Protocols

Product Specific Protocols
WB protocol for Rootletin antibody 55485-1-APDownload protocol
IF protocol for Rootletin antibody 55485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols