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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100B Monoclonal antibody, PBS Only

S100B Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10F4

Cat no : 66616-1-PBS

Synonyms

S100 beta, S-100 protein beta chain, S100 calcium-binding protein B, Protein S100-B, Protein S100 B

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using S100B Monoclonal antibody (66616-1-Ig)

    WB analysis of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using S100B Monoclonal antibody (66616-1-Ig)

    WB analysis of C6 using 66616-1-Ig (same clone as 66616-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using S100B Monoclonal antibody (66616-1-Ig)

    WB analysis of pig brain using 66616-1-Ig (same clone as 66616-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of human malignant melanoma using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of human malignant melanoma using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of human appendicitis using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66616-1-Ig (S100 beta antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of human appendicitis using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66616-1-Ig (S100 beta antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using S100B Monoclonal antibody (66616-1-Ig)

    IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using S100B Monoclonal antibody (66616-1-Ig)

    IF Staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using S100 Beta antibody (66616-1-Ig, Clone: 1G10F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66616-1-PBS targets S100B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100B fusion protein Ag7440 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein B
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC001766
Gene symbol S100 Beta
Gene ID (NCBI) 6285
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.