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  • Featured Product
  • KD/KO Validated

S100A10 Polyclonal antibody

S100A10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11250-1-AP

Synonyms

Calpactin-1 light chain, Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using S100A10 Polyclonal antibody (11250-1-AP)

    WB analysis using 11250-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using S100A10 Polyclonal antibody (11250-1-AP)

    WB analysis using 11250-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using S100A10 Polyclonal antibody (11250-1-AP)

    WB analysis using 11250-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using S100A10 Polyclonal antibody (11250-1-AP)

    WB analysis of human lung using 11250-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using S100A10 Polyclonal antibody (11250-1-AP)

    WB analysis using 11250-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human lung cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human lung cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human colon using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11250-1-AP (S100A10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human colon using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human gliomas using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human gliomas using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human cervical cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human cervical cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human prostate hyperplasia using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human prostate hyperplasia using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human skin using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human skin using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human pancreas cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human pancreas cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human lung cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human lung cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human pancreas cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Polyclonal antibody (11250-1-AP)

    IHC staining of human pancreas cancer using 11250-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using S100A10 Polyclonal antibody (11250-1-AP)

    IF Staining of HepG2 using 11250-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using S100A10 antibody (11250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using S100A10 Polyclonal antibody (11250-1-AP)

    IF Staining of HeLa using 11250-1-AP

    Immunofluorescent analysis of HeLa cells using 11250-1-AP (S100A10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, HT-29 cells, human lung tissue, HaCaT cells
Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue, human gliomas tissue, human normal colon, human pancreas cancer tissue, human prostate hyperplasia tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11250-1-AP targets S100A10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A10 fusion protein Ag1779 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A10
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC015973
Gene symbol S100A10
Gene ID (NCBI) 6281
RRIDAB_2269906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Protocols

Product Specific Protocols
WB protocol for S100A10 antibody 11250-1-APDownload protocol
IHC protocol for S100A10 antibody 11250-1-APDownload protocol
IF protocol for S100A10 antibody 11250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.

Authors - Yunwei Lou
View Article
ratIF

JCI Insight

Fabry disease Schwann cells release p11 to induce sensory neuron hyperactivity

Authors - Tyler B Waltz
View Article
mouseWB,IP

Diabetes

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Authors - Hua Qu
  • KD Validated
View Article
humanIF

J Nanobiotechnology

Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

Authors - Xiao Zhai
View Article
humanWB

J Med Chem

5α-Epoxyalantolactone Inhibits Metastasis of Triple-Negative Breast Cancer Cells by Covalently Binding a Conserved Cysteine of Annexin A2.

Authors - Mingming Wei
View Article
human,mouseIHC

Cancers (Basel)

S100A10 Has a Critical Regulatory Function in Mammary Tumor Growth and Metastasis: Insights Using MMTV-PyMT Oncomice and Clinical Patient Sample Analysis.

Authors - Alamelu G Bharadwaj
  • KO Validated
View Article