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KD/KO Validated

S100A10 Polyclonal antibody

Name: S100A10 Polyclonal antibody
Brand: Proteintech
SKU: 11250-1-AP

S100A10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11250-1-AP

Synonyms

Calpactin-1 light chain, Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 11250-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11250-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11250-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11250-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11250-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human lung cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).
IHC staining of human colon using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11250-1-AP (S100A10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human gliomas using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 11250-1-AP (S100A10 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human cervical cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate hyperplasia using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human skin using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human skin using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).
IHC staining of human pancreas cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human pancreas cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).
IHC staining of human prostate hyperplasia using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11250-1-AP (S100A10 antibody) at dilution of 1:400 (under 40x lens).
IHC staining of human lung cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human pancreas cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas cancer using 11250-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 11250-1-AP
Immunofluorescent analysis of HeLa cells using 11250-1-AP (S100A10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 11250-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using S100A10 antibody (11250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 11250-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A10 Polyclonal antibody (11250-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 11250-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, HT-29 cells, human lung tissue, HaCaT cells
Positive IHC detected in	human lung cancer tissue, human cervical cancer tissue, human gliomas tissue, human normal colon, human pancreas cancer tissue, human prostate hyperplasia tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:100-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 30 publications below
IHC	See 7 publications below
IF	See 22 publications below
IP	See 3 publications below
ELISA	See 1 publications below
FC	See 1 publications below

Product Information

11250-1-AP targets S100A10 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	S100A10 fusion protein Ag1779 相同性解析による交差性が予測される生物種
Full Name	S100 calcium binding protein A10
Calculated molecular weight	11 kDa
Observed molecular weight	11 kDa
GenBank accession number	BC015973
Gene symbol	S100A10
Gene ID (NCBI)	6281
RRID	AB_2269906
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Protocols

Product Specific Protocols
WB protocol for S100A10 antibody 11250-1-AP	Download protocol
IHC protocol for S100A10 antibody 11250-1-AP	Download protocol
IF protocol for S100A10 antibody 11250-1-AP	Download protocol
FC protocol for S100A10 antibody 11250-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection. Authors - Yunwei Lou View Article
rat	IF	JCI Insight Fabry disease Schwann cells release p11 to induce sensory neuron hyperactivity Authors - Tyler B Waltz View Article
mouse	WB,IF	Phytomedicine Withaferin A protects against epilepsy by promoting LCN2-mediated astrocyte polarization to stopping neuronal ferroptosis Authors - Zixian Zhou View Article
mouse	WB,IP	Diabetes Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease. Authors - Hua Qu KD Validated View Article
human	IF	J Nanobiotechnology Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury Authors - Xiao Zhai View Article
human	WB	J Med Chem 5α-Epoxyalantolactone Inhibits Metastasis of Triple-Negative Breast Cancer Cells by Covalently Binding a Conserved Cysteine of Annexin A2. Authors - Mingming Wei View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100A10 Polyclonal antibody

S100A10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 11250-1-AP

WB analysis using 11250-1-AP

WB analysis using 11250-1-AP

WB analysis of human lung using 11250-1-AP

WB analysis using 11250-1-AP

IHC staining of human lung cancer using 11250-1-AP

IHC staining of human lung cancer using 11250-1-AP

IHC staining of human colon using 11250-1-AP

IHC staining of human colon using 11250-1-AP

IHC staining of human gliomas using 11250-1-AP

IHC staining of human gliomas using 11250-1-AP

IHC staining of human cervical cancer using 11250-1-AP

IHC staining of human cervical cancer using 11250-1-AP

IHC staining of human prostate hyperplasia using 11250-1-AP

IHC staining of human skin using 11250-1-AP

IHC staining of human skin using 11250-1-AP

IHC staining of human pancreas cancer using 11250-1-AP

IHC staining of human pancreas cancer using 11250-1-AP

IHC staining of human prostate hyperplasia using 11250-1-AP

IHC staining of human lung cancer using 11250-1-AP

IHC staining of human lung cancer using 11250-1-AP

IHC staining of human pancreas cancer using 11250-1-AP

IHC staining of human pancreas cancer using 11250-1-AP

IF Staining of HeLa using 11250-1-AP

IF Staining of HepG2 using 11250-1-AP

FC experiment of HeLa using 11250-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.

Fabry disease Schwann cells release p11 to induce sensory neuron hyperactivity

Withaferin A protects against epilepsy by promoting LCN2-mediated astrocyte polarization to stopping neuronal ferroptosis

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury

5α-Epoxyalantolactone Inhibits Metastasis of Triple-Negative Breast Cancer Cells by Covalently Binding a Conserved Cysteine of Annexin A2.

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