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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100A10 Monoclonal antibody, PBS Only

S100A10 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B3F9

Cat no : 66227-1-PBS

Synonyms

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    WB analysis of human lung using 66227-1-Ig (same clone as 66227-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 66227-1-Ig (S100A10 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human stomach cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human liver cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human liver cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human cervical cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human cervical cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human pancreas cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human pancreas cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Monoclonal antibody (66227-1-Ig)

    IHC staining of human pancreas cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using S100A10 Monoclonal antibody (66227-1-Ig)

    IF Staining of A431 using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using S100A10 Monoclonal antibody (66227-1-Ig)

    IF Staining of Saos-2 using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66227-1-PBS targets S100A10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100A10 fusion protein Ag23596 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A10
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC015973
Gene symbol S100A10
Gene ID (NCBI) 6281
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.