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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using S100A10 Recombinant antibody (81017-1-RR)

    WB analysis of A431 using 81017-1-RR (same clone as 81017-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 81017-1-RR (S100A10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human colon using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81017-1-RR (S100A10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human lung cancer using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human lung cancer using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of mouse lung using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of mouse lung using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of mouse skin using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of mouse skin using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human pancreas cancer using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A10 Recombinant antibody (81017-1-RR)

    IHC staining of human pancreas cancer using 81017-1-RR (same clone as 81017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81017-1-RR (S100A10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81017-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81017-1-RR

    Affinity and Kinetic Characterization of 81017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81017-1-RR against Human S100A10 were performed. The affinity constant is 24.5 nM.

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S100A10 Recombinant antibody, PBS Only

S100A10 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81017-1-PBS
Clone No.8G23

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG, ANX2L

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81017-1-PBS targets S100A10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100A10 fusion protein Ag1779 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A10
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC015973
Gene Symbol S100A10
Gene ID (NCBI) 6281
RRIDAB_2918925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60903
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.