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- KD/KO Validated
S100A11 Monoclonal antibody
S100A11 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1A3F1
Cat no : 60024-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | T-47D cells, A431 cells, BxPC-3 cells, DU 145 cells |
| Positive IHC detected in | human lung cancer tissue, human ovary tumor tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
60024-1-Ig targets S100A11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | S100A11 fusion protein Ag0343 相同性解析による交差性が予測される生物種 |
| Full Name | S100 calcium binding protein A11 |
| Calculated molecular weight | 105 aa, 12 kDa |
| Observed molecular weight | 12 kDa |
| GenBank accession number | BC001410 |
| Gene symbol | S100A11 |
| Gene ID (NCBI) | 6282 |
| RRID | AB_2238821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
S100A11, also named as MLN 70, Calgizzarin and S100C, belongs to the S-100 family. It facilitates the differentiation and the cornification of keratinocytes. The naming of S100A11 systematically arises from its membership of the S100 protein family, named after their solubility in 100% saturated ammonium sulfate solution. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for S100A11 antibody 60024-1-Ig | Download protocol |
| IHC protocol for S100A11 antibody 60024-1-Ig | Download protocol |
| IF protocol for S100A11 antibody 60024-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics S100A11 mediates hypoxia-induced mitogenic factor (HIMF)-induced smooth muscle cell migration, vesicular exocytosis, and nuclear activation.
| ||
Talanta Effective enrichment of cholangiocarcinoma secretomes using the hollow fiber bioreactor culture system. | ||
Nat Commun Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis | ||
Phytomedicine Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity | ||
Cell Rep Extracellular-vesicle-packaged S100A11 from osteosarcoma cells mediates lung premetastatic niche formation by recruiting gMDSCs
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