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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100A16 Recombinant antibody, PBS Only

S100A16 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E1

Cat no : 82923-1-PBS

Synonyms

Protein S100-A16, Protein S100 F, Protein S100 A16, Aging-associated gene 13 protein, AAG13

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using S100A16 Recombinant antibody (82923-1-RR)

    WB analysis of A431 using 82923-1-RR (same clone as 82923-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 82923-1-RR (S100A16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82923-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using S100A16 Recombinant antibody (82923-1-RR)

    FC experiment of A431 using 82923-1-RR (same clone as 82923-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A16 Recombinant antibody (82923-1-RR, Clone:3E1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82923-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82923-1-PBS targets S100A16 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100A16 fusion protein Ag2023 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A16
Calculated molecular weight 103 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC019099
Gene symbol S100A16
Gene ID (NCBI) 140576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).