• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using S100A2 Polyclonal antibody (15454-1-AP)

    WB analysis using 15454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15454-1-AP (S100A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using S100A2 Polyclonal antibody (15454-1-AP)

    WB analysis using 15454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15454-1-AP (S100A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human bowen disease using S100A2 Polyclonal antibody (15454-1-AP)

    IHC staining of human bowen disease using 15454-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15454-1-AP (S100A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using S100A2 Polyclonal antibody (15454-1-AP)

    IHC staining of human bowen disease using 15454-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15454-1-AP (S100A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using S100A2 Polyclonal antibody (15454-1-AP)

    IF Staining of A431 using 15454-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using S100A2 antibody (15454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using S100A2 Polyclonal antibody (15454-1-AP)

    FC experiment of A431 using 15454-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.4 ug S100A2 Polyclonal antibody (15454-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

S100A2 Polyclonal antibody

S100A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15454-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

S100 calcium-binding protein A2, Protein S-100L, Protein S100-A2, Protein S100 A2, Protein S 100L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHaCaT cells, HeLa cells
Positive IHC detected inhuman bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15454-1-AP targets S100A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A2 fusion protein Ag7719 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A2
Calculated molecular weight 11 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC002829
Gene Symbol S100A2
Gene ID (NCBI) 6273
RRIDAB_3669209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29034
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A2 (S100 calcium-binding protein A2) is also named as S100L and CAN19. S100A2, a member of the S100 protein family, is more highly expressed in endometrial carcinoma tissues than in normal tissues and was correlated with worse survival (PMID: 35042454). S100A2 has a tumor suppressor function. S100A2 is highly upregulated in the epidermis under inflammatory conditions and in drug eruptions, in addition to inflammatory skin diseases such as atopic dermatitis and psoriasis (PMID: 35885660).

Protocols

Product Specific Protocols
WB protocol for S100A2 antibody 15454-1-APDownload protocol
IHC protocol for S100A2 antibody 15454-1-APDownload protocol
IF protocol for S100A2 antibody 15454-1-APDownload protocol
FC protocol for S100A2 antibody 15454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols