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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100A5 Polyclonal antibody

S100A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17924-1-AP

Synonyms

S100D, S100 calcium-binding protein A5, Protein S-100D, Protein S100-A5, Protein S100 A5

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using S100A5 Polyclonal antibody (17924-1-AP)

    WB analysis of HepG2 using 17924-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using S100A5 Polyclonal antibody (17924-1-AP)

    WB analysis of human liver using 17924-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using S100A5 Polyclonal antibody (17924-1-AP)

    WB analysis of SH-SY5Y using 17924-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using S100A5 Polyclonal antibody (17924-1-AP)

    WB analysis of human kidney using 17924-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human meningioma tissue using S100A5 Polyclonal antibody (17924-1-AP)

    IHC staining of human meningioma using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human meningioma tissue using S100A5 Polyclonal antibody (17924-1-AP)

    IHC staining of human meningioma using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using S100A5 Polyclonal antibody (17924-1-AP)

    IHC staining of human brain using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17924-1-AP (S100A5 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using S100A5 Polyclonal antibody (17924-1-AP)

    IHC staining of human brain using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17924-1-AP (S100A5 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human meningioma tissue using S100A5 Polyclonal antibody (17924-1-AP)

    IHC staining of human meningioma using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human meningioma tissue using S100A5 Polyclonal antibody (17924-1-AP)

    IHC staining of human meningioma using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:400 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using S100A5 Polyclonal antibody (17924-1-AP)

    IF Staining of HepG2 using 17924-1-AP

    Immunofluorescent analysis of HepG2 cells using 17924-1-AP (S100A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, human kidney tissue, human liver tissue, SH-SY5Y cells
Positive IHC detected inhuman meningioma tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17924-1-AP targets S100A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A5 fusion protein Ag12342 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A5
Calculated molecular weight 110 aa, 13 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC093955
Gene symbol S100A5
Gene ID (NCBI) 6276
RRIDAB_2183794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for S100A5 antibody 17924-1-APDownload protocol
IHC protocol for S100A5 antibody 17924-1-APDownload protocol
IF protocol for S100A5 antibody 17924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Adv Sci (Weinh)

S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8+ T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma

Authors - Huihuang Li
View Article
mouseIHC

Mol Cell Proteomics

The Membrane Proteome of Sensory Cilia to the Depth of Olfactory Receptors.

Authors - Katja Kuhlmann
View Article