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  • Featured Product
  • KD/KO Validated

S100A6 Polyclonal antibody, PBS Only

S100A6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10245-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using S100A6 Polyclonal antibody (10245-1-AP)

    WB analysis of HeLa using 10245-1-AP (same clone as 10245-1-PBS)

    WB result of S100A6 antibody (10245-1-AP, 1:30000) with si-Control and si-S100A6 transfected HeLa cells.. This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using S100A6 Polyclonal antibody (10245-1-AP)

    WB analysis using 10245-1-AP (same clone as 10245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10245-1-AP (S100A6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using S100A6 Polyclonal antibody (10245-1-AP)

    WB analysis of A549 using 10245-1-AP (same clone as 10245-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 10245-1-AP (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using S100A6 Polyclonal antibody (10245-1-AP)

    IP experiment of A549 using 10245-1-AP (same clone as 10245-1-PBS)

    IP result of anti-S100A6 (IP:10245-1-AP, 4ug; Detection:10245-1-AP 1:5000) with A549 cells lysate 840 ug. This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human stomach cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human stomach cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human pancreas cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human pancreas cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human stomach cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human stomach cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human colon cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human colon cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human liver cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human liver cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human liver cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using S100A6 Polyclonal antibody (10245-1-AP)

    IHC staining of human liver cancer using 10245-1-AP (same clone as 10245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using S100A6 Polyclonal antibody (10245-1-AP)

    IF Staining of MCF-7 using 10245-1-AP (same clone as 10245-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using S100A6 antibody (10245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using S100A6 Polyclonal antibody (10245-1-AP)

    IF Staining of MCF-7 using 10245-1-AP (same clone as 10245-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using S100A6 antibody 10245-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using S100A6 Polyclonal antibody (10245-1-AP)

    FC experiment of MCF-7 using 10245-1-AP (same clone as 10245-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human S100A6 (10245-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10245-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10245-1-PBS targets S100A6 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A6 fusion protein Ag0385 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A6
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC001431
Gene symbol S100A6
Gene ID (NCBI) 6277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.