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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using S100A7/Psoriasin Polyclonal antibody (26656-1-AP)

    WB analysis of MCF-7 using 26656-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-468 cells using S100A7/Psoriasin Polyclonal antibody (26656-1-AP)

    WB analysis of MDA-MB-468 using 26656-1-AP

    MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using S100A7/Psoriasin Polyclonal antibody (26656-1-AP)

    IHC staining of human oesophagus cancer using 26656-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using S100A7/Psoriasin Polyclonal antibody (26656-1-AP)

    IHC staining of human oesophagus cancer using 26656-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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S100A7/Psoriasin Polyclonal antibody

S100A7/Psoriasin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26656-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Protein S100 A7, PSOR1, Psoriasin, S100A7, S100A7/Psoriasin, S100A7c

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-468 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26656-1-AP targets S100A7/Psoriasin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A7/Psoriasin fusion protein Ag24717 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A7
Calculated molecular weight 101 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC034687
Gene Symbol S100A7/Psoriasin
Gene ID (NCBI) 6278
RRIDAB_2880592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A7 (psoriasin), a member of the S100 protein family, is a well-known antimicrobial peptide and a signaling molecule which regulates cellular function and is expressed in many squamous cell carcinomas (SCCs), such as SCC of the skin. It is an EF-hand type calcium binding protein localized in epithelial cells, regulates cell proliferation and differentiation. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.

Protocols

Product Specific Protocols
WB protocol for S100A7/Psoriasin antibody 26656-1-APDownload protocol
IHC protocol for S100A7/Psoriasin antibody 26656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols