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S100A8 抗体 12 文献

Rabbit Polyclonal| カタログ番号: 15792-1-AP

注目製品 KD/KO 検証済

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IHC, IF, FC
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Images See all

S100A8 Antibody WB THP-1 cells 15792-1-AP

THP-1 cells were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

S100A8 Antibody IHC human stomach cancer tissue 15792-1-AP

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

S100A8 Antibody FC RAW 264.7 cells 15792-1-AP

1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human S100A8 (15792-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

種特異性:
human, mouse, rat
WB ポジティブ:
THP-1 cells, A549 cells, COLO 320 cells
IHC ポジティブ:
human stomach cancer tissue
FC ポジティブ:
RAW 264.7 cells
推奨希釈倍率:
WB : 1:500-1:1000
IHC : 1:200-1:800

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
S100A8 fusion protein Ag8500
正式名:
S100 calcium binding protein A8
計算分子量:
93 aa, 11 kDa
検出分子量:
11 kDa
GenBank accession number:
BC005928
Gene ID (NCBI):
6279
遺伝子シンボル:
S100A8
別名:
60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A

背景説明

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).


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