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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100A8 Monoclonal antibody, PBS Only

S100A8 Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G11C8

Cat no : 66853-1-PBS

Synonyms

60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A8 Monoclonal antibody (66853-1-Ig)

    IHC staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A8 Monoclonal antibody (66853-1-Ig)

    IHC staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using S100A8 Monoclonal antibody (66853-1-Ig)

    IHC staining of human cervical cancer using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using S100A8 Monoclonal antibody (66853-1-Ig)

    IHC staining of human cervical cancer using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using S100A8 Monoclonal antibody (66853-1-Ig)

    IHC staining of human breast cancer using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using S100A8 Monoclonal antibody (66853-1-Ig)

    IHC staining of human breast cancer using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using S100A8 Monoclonal antibody (66853-1-Ig)

    IF Staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using S100A8 antibody (66853-1-Ig, Clone: 1G11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using S100A8 Monoclonal antibody (66853-1-Ig)

    IF Staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using S100A8 antibody (66853-1-Ig, Clone: 1G11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66853-1-PBS targets S100A8 in IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen S100A8 fusion protein Ag8517 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A8
Calculated molecular weight 93 aa, 11 kDa
GenBank accession numberBC005928
Gene symbol S100A8
Gene ID (NCBI) 6279
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.