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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100A9 Recombinant antibody, PBS Only (Capture)

S100A9 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240539G9

Cat no : 83578-2-PBS

Synonyms

CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using S100A9 Recombinant antibody (83578-2-RR)

    WB analysis of MCF-7 using 83578-2-RR (same clone as 83578-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using S100A9 Recombinant antibody, PBS Only (Capture) (83578-2-PBS)

    WB analysis using 83578-2-PBS

    Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-PBS (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using S100A9 Recombinant antibody (83578-2-RR)

    WB analysis using 83578-2-RR (same clone as 83578-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using S100A9 Recombinant antibody (83578-2-RR)

    IF Staining of A549 using 83578-2-RR (same clone as 83578-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using S100A9 Recombinant antibody (83578-2-RR)

    IF Staining of HeLa using 83578-2-RR (same clone as 83578-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00569-3

    Cytometric bead array standard curve of MP00569-3

    Cytometric bead array standard curve of MP00569-3, S100A9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83578-2-PBS. Detection antibody: 83578-6-PBS. Standard: Ag25764. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83578-2-RR

    Affinity and Kinetic Characterization of 83578-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83578-2-RR against Human S100A9 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inTHP-1 cells, MCF-7 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83578-2-PBS targets S100A9 as part of a matched antibody pair:

MP00569-3: 83578-2-PBS capture and 83578-6-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100A9 fusion protein Ag25764 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A9
Calculated molecular weight 13 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC047681
Gene symbol S100A9
Gene ID (NCBI) 6280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Protocols

Product Specific Protocols
WB protocol for S100A9 antibody 83578-2-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols