S100P Polyclonal antibody

Name: S100P Polyclonal antibody
Brand: Proteintech
SKU: 11803-1-AP

S100P Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11803-1-AP

Synonyms

S100 calcium-binding protein P, S100 calcium binding protein P, Protein S100-P, Protein S100-E, Protein S100 P

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HeLa using 11803-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11803-1-AP (S100P antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11803-1-AP
HuH-7 cells were subjected to SDS PAGE followed by western blot with 11803-1-AP (S100P antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11803-1-AP (S100P antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11803-1-AP (S100P antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11803-1-AP (S100P antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human urothelial carcinoma using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung cancer using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung cancer using 11803-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 11803-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100P antibody (11803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 11803-1-AP
Immunofluorescent analysis of HepG2 cells, using S100P antibody 11803-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 11803-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HuH-7 cells
Positive IHC detected in	human pancreas cancer tissue, human colon cancer tissue, human lung cancer tissue, human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:6000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 6 publications below

Product Information

11803-1-AP targets S100P in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	S100P fusion protein Ag2379 相同性解析による交差性が予測される生物種
Full Name	S100 calcium binding protein P
Calculated molecular weight	95 aa, 11 kDa
Observed molecular weight	10 kDa
GenBank accession number	BC006819
Gene symbol	S100P
Gene ID (NCBI)	6286
RRID	AB_2184577
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.

Protocols

Product Specific Protocols
WB protocol for S100P antibody 11803-1-AP	Download protocol
IHC protocol for S100P antibody 11803-1-AP	Download protocol
IF protocol for S100P antibody 11803-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC	Adv Sci (Weinh) RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation Authors - Yu Sun View Article
human	WB, IHC	Am J Cancer Res High expression of S100P is associated with unfavorable prognosis and tumor progression in patients with epithelial ovarian cancer. Authors - Xiangyu Wang View Article
human	IHC	Proteomics Clin Appl Identification of distinctive protein expression patterns in colorectal adenoma. Authors - F Lam Francis F View Article
human	IHC	Diagn Pathol Basal cell adenocarcinoma of the salivary gland: a morphological and immunohistochemical comparison with basal cell adenoma with and without capsular invasion. Authors - Jung Min Jung MJ View Article
human	WB	Exp Ther Med Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma. Authors - Chengyu Wang View Article
human	WB	Technol Cancer Res Treat LHPP Inhibits the Viability, Migration, and Proliferation of PDAC Cells and Significantly Affects the Expression of SDC1 and S100p Authors - Zhaozhi Xia View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100P Polyclonal antibody

S100P Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HeLa using 11803-1-AP

WB analysis using 11803-1-AP

IHC staining of human pancreas cancer using 11803-1-AP

IHC staining of human pancreas cancer using 11803-1-AP

IHC staining of human bowen disease using 11803-1-AP

IHC staining of human colon cancer using 11803-1-AP

IHC staining of human lung cancer using 11803-1-AP

IHC staining of human lung cancer using 11803-1-AP

IHC staining of human urothelial carcinoma using 11803-1-AP

IHC staining of human urothelial carcinoma using 11803-1-AP

IHC staining of human colon cancer using 11803-1-AP

IHC staining of human colon cancer using 11803-1-AP

IHC staining of human lung cancer using 11803-1-AP

IHC staining of human lung cancer using 11803-1-AP

IF Staining of HeLa using 11803-1-AP

IF Staining of HepG2 using 11803-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation

High expression of S100P is associated with unfavorable prognosis and tumor progression in patients with epithelial ovarian cancer.

Identification of distinctive protein expression patterns in colorectal adenoma.

Basal cell adenocarcinoma of the salivary gland: a morphological and immunohistochemical comparison with basal cell adenoma with and without capsular invasion.

Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma.

LHPP Inhibits the Viability, Migration, and Proliferation of PDAC Cells and Significantly Affects the Expression of SDC1 and S100p