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Validation Data Gallery

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  • Western Blot (WB) analysis of BXPC-3 cells using S100P Monoclonal antibody (67485-1-Ig)

    WB analysis of BXPC-3 using 67485-1-Ig (same clone as 67485-1-PBS)

    BXPC-3 cells were subjected to SDS PAGE followed by western blot with 67485-1-Ig (S100P antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human pancreas cancer using 67485-1-Ig (same clone as 67485-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human pancreas cancer using 67485-1-Ig (same clone as 67485-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human urothelial carcinoma using 67485-1-Ig (same clone as 67485-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human urothelial carcinoma using 67485-1-Ig (same clone as 67485-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using S100P Monoclonal antibody (67485-1-Ig)

    IF Staining of HeLa using 67485-1-Ig (same clone as 67485-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100P antibody (67485-1-Ig, Clone: 1G6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51168-2

    Cytometric bead array standard curve of MP51168-2

    Cytometric bead array standard curve of MP51168-2, S100P Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67485-1-PBS. Detection antibody: 67485-4-PBS. Standard:Ag19115. Range: 6.25-100 ng/mL.

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S100P Monoclonal antibody, PBS Only (Capture)

S100P Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67485-1-PBS
Clone No.1G6C3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

S100 calcium binding protein P, Protein S100-P, Protein S100-E, Protein S100 P, Migration-inducing gene 9 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67485-1-PBS targets S100P as part of a matched antibody pair:

MP51168-2: 67485-1-PBS capture and 67485-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen S100P fusion protein Ag19115 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein P
Calculated molecular weight 95 aa, 11 kDa
Observed molecular weight 10 KDa
GenBank accession numberBC006819
Gene Symbol S100P
Gene ID (NCBI) 6286
RRIDAB_2882712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25815
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.